QBC for the diagnosis of human and canine american visceral leishmaniasis: preliminary data.

Application of Recombinant Proteins for Serodiagnosis of Visceral Leishmaniasis in Humans and Dogs

Visceral leishmaniasis (VL) is a zoonotic disease caused by leishmania species. Dogs are considered to be the main reservoir of VL. A number of methods and antigen-based assays are used for the diagnosis of leishmaniasis. However, currently available methods are mainly based on direct examination of tissues for the presence of parasites, which is highly invasive. A variety of serological tests ...

A Leishmania infantum FML-ELISA for the Detection of Symptomatic and Asymptomatic Canine Visceral Leishmaniasis in an Endemic Area of Iran

Background: Visceral leishmaniasis (VL) is caused by Leishmania infantum in Mediterranean basin and is an endemic disease in some parts of Iran. Canines are the main reservoirs of VL in most of the endemic areas. Different serological methods have been introduced for diagnosis of canine visceral leishmaniasis (CVL). Objective: In this survey a Fucose-Mannose Ligand (FML) ELISA, using native L. ...

Field Trial of Autoclaved Leishmania Vaccines for Control of Canine Visceral Leishmaniasis in Meshkin-Shahr, Iran

Preparation and evaluation of latex agglutination technique for diagnosis and seroepidemiological studies of visceral leishmaniasis

in this study which has been carried out from 1996 until 1997 soluble latex agglutination antigen was prepared from L, donovani,sudan(s-1) by culturing in special media,harvesting,and later solubilizing by freezing and thawing.the antigen were futher prepared by high speed centrifugation.after measurement of the protein (lowry method) concentration of the antigen was set at 2.2 mg/ml,the antige...

Seroepidemiological investigation of visceral leishmaniasis in dogs of Ahvaz district, Iran

Visceral leishmaniasis is a parasitic infectious disease from dogs and canids to human that is caused by protozoans of the genus Leishmania. Information on the prevalence of canine leishmaniasis is necessary to define control measures for zoonotic leishmaniasis. This seroepidemiological survey was performed in dogs from Ahvaz district using by DAT and ELISA. Blood was randomly collected in 38 p...